Main Field of Research, Abstract
Genotoxic stress, as ionizing radiation (IR), is a life-threatening event for organisms as it alters the content and organization of the genetic material. The cellular responses to this danger are diverse and culminate in the activation of cell-cycle checkpoints and DNA repair pathways. Using Reverse Phase Protein Microarrays (RPPAs) we would like to understand the complex signaling network, activated in response to genotoxic stress, by looking at the phosphorylaiton status of key cellular signaling nodes. The outcome of this study will allow to identify potential key denominators with impact on diagnostic analyses of environmental influences on human or medically prescribed tumor radiotherapies and their prognosis.
Main Fields of Research, Keywords Primary human fibroblasts, DNA damage response, proteomics, signaling, radiosensitization
Special Techniques and Equipment
Reverse phase protein microarrays (RPPAs), fluorescence microscopy, Western Blotting (WB), FACS, cell viability assays, statistical analysis